Abstract：Rapid sweating during high-intensity exercise training or competition leads to dehydration and loss of electrolytes, thereby result in decreasing exercise performance. The purpose of this study was to investigate the effects of deep ocean minerals supplementation on the balance of hydration status following dehydration. The crossover experimental design was used in this study. Eight young male subjects received either placebo (PLA) or deep ocean minerals (DOM) trails. Each trail was separated and repeated the same protocols after one week washout period. Subject performed a single bout of moderate-high intensity exercise in a high temperature environment in order to loss 3% body weight. After exercise subjects received either deep ocean minerals or PLA supplements (equivalent to 1.5 times of body weight losses) to replace their fluid losses. Body weight, urine color, red blood cells and hemotocrit were measured 24 and 48 hours following dehydration. We found that the loss in body weight after dehydration was regained to pre-dehydration level within 24 hours in both trails. The urine volume in DOM trial was significantly increased within 4 hours compared to PLA trail. The red blood cell number and hemotocrit after dehydration were significantly lower than pre-dehydration level in both trials; however no significant difference between PLA and DOM trails. The DOM trail significantly decreased the urine color compared to the PLA trail. Our results demonstrated that supplementation of deep ocean minerals can increase the recovery status following intense exercise. This study suggests that deep ocean minerals supplementation is effective to promote the rehydration status. (Full text)