Abstract：Previous studies have demonstrated that chronic dehydroepiandrosterone (DHEA) supplementation could improve glucose disposal and metabolic status. However, glucose absorption ability with response to acute and chronic exercise training along with DHEA supplementation is currently unclear. In this study, we investigated the effect of resistance training along with DHEA supplementation on glucose tolerance and hormone response. Nineteen healthy females (age: 39.4±1.54) were recruited in this study and all the subjects were performed the maximal repetition test (RM) to determine the basal maximal strength before the treatment. Subjects were divided into three groups; DHEA supplementation (n=5; 50 mg, daily), resistance training (n=6; 70% of RM) and resistance training plus DHEA supplementation (n=8). Blood samples were collected at study beginning, after 3 days and 8 weeks to determine the hormone response, glucose area under curve (GAUC) and insulin area under curve (IAUC). Body composition and muscular strength were also measured. In the results we observed subjects' sit-up ability was increased in resistance training group. The concentration of cortisol in trained group was significantly increased only after 3 days. However, the concentration of testosterone were significantly increased in resistance training plus DHEA supplementation group compared to resistance training group, but GAUC and IAUC were trendily decreased. After 8-week treatment, the level of cortisol was significantly lower than baseline. The major findings of this study were that DHEA supplementation in the early phase of resistance training could improve glucose tolerance and insulin sensitivity. However, this beneficial effect was not reflected in the chronic treatment. (Full text)