Abstract：Triathlon competition is a high-intensity endurance sport. During the competition, excessive sweating may result in the loss of electrolytes, such as potassium and sodium that would affect the next day's competition performance of participants. Therefore, this study investigated whether high mineral sports drink supplementation can accelerate the physical recovery within 24 h after high-intensity exercise. Seventeen triathletes were recruited for this study, and a crossover design was adopted. Each athlete performed a high-intensity exercise 2 times separated by a 2 days interval. During each test, participants rode a bike for 1 h at 70% maximal oxygen consumption, and consumed a 600 ml of high mineral or low mineral sports drink. Athletic performances were assessed using maximal running performance test and sports ability tests. After 24 h of physical recovery, the athletic performances of each athlete were re-assessed using the same tests. The results showed that 24 h after high-intensity exercise, high mineral sports drink supplementation produced better physical recovery in maximal running performance compared with low mineral sports drink. The malondialdehyde levels were significantly lower right after high-intensity exercise and also after 24 h of recovery with high mineral sports drink. The red blood cell concentration and hematocrit were significantly lower in athletes with high mineral sports drink after 24 h of physical recovery compared with low mineral sports drink supplementation. Total blood flow in prefrontal cortex was higher from 150 to 230 s in athletes having high mineral sports drink compared with low mineral sports drink. Oxyhemoglobin was higher from 150 to 180 s in athletes having low mineral sports drink, but it was higher from 210 to 260 s in athletes having high mineral sports drink. In conclusion, supplementation of high mineral sports drink during high-intensity exercise can reduce the oxidative stress and accelerate 24 h of physical recovery in triathletes. (Full text)