Abstract：The purpose of this study was to investigate the effects of different home exercise programs on blood glucose regulation in middle-aged women. The participants were middle-aged women with (n = 57) prediabetes, whose fasting blood glucose values were between 100 and 125 mg/dL. All participants were randomly divided into 2 groups. Thirty participants in the regular aerobic exercise group performed a 60-min cycling exercise, three days per week for a period of 24 weeks. The aerobic cycling exercise program consisted of 10 min warm-up, 40 min moderate-intensity aerobic exercise, and 10 min relaxing exercise. In another group, 27 participants performed daily walking program with 8,000 steps per day for 24 weeks. Total steps were recorded with a smart bracelet. The results showed that the body weight, waist circumference, fasting blood glucose, and glycated hemoglobin (HbA1c) of the participants were significantly improved by the regular aerobic exercise program. The daily walking exercise also significantly improved the responses of fasting blood glucose and HbA1c, which was better than that of aerobic exercise in middle-aged women. Therefore, we concluded that daily walking habit improved HbA1c levels effectively, and thereby prevents the prediabetes in middle-aged women. (Full text)